Uncontrollable 16 year old Tabby McCallum (Joan Freeman) is chastised by the Virginian for shooting a shiloh steer. In retaliation she tries to burn down the Shiloh barn but is accidentally shot by a Shiloh hand, Sam Hicks (Charles Aidman). Tabby's brother Bruce McCallum (Carl Reindell), who is from the same mould as Tabby, claims she was deliberately shot while her father, Tucker (Charles Bickford), thinks her boy friend, Dan Flood (Bert Brinckerhof), started the fire. The result is a spiralling series of events which eventually lead to murder.

In self-defence, Trampas is forced to shoot Big John Belden (Charles McGraw), who took exception to Trampas dancing with his his son Martin's (Fabian) fiancee. John is left a paraplegic and he encourages his son to take revenge on Trampas for the family honor even though Martin is, in direct contrast to his father, very mild mannered. Trampas is afraid he will have to kill Martin but their feud is interrupted by an encounter with Moses, a fierce lengendary bear.

Accompanied by her secretary (Melinda Plowman), opera singer Elena (Yvonne DeCarlo) comes to Medicine Bow to perform. Judge Garth recognizes here as someone he knew years before and is clearly attracted to her. A man named Carl Elston arrives in town and is shot by Elena who claims he is a stranger who tried to force his attentions on her. However, Sheriff Abbott (Ross Elliott) discovers evidence that Elena knew Carl, and that he was her manager and husband. The Judge is forced to defend Elena in court and uncover the truth.

Trampas' Uncle Josh (Harry Shannon) is murdered during an holdup by Wismer (Leonard Nimoy) and Judson (William Bryant). On their trail, Trampas is eventually forced to illegally enter Apache territory where the two robbers had made a gold strike. He is joined on his quest by a guide who is after the gold (Michael Pate), a woman (Peggy McCay) who is looking for her husband who it turns out is Judson, and an army doctor who is attempting to escape court martial (DeForest Kelly).

Randy and Betsy explore a cave and find a number of gold coins and a human skeleton, a find which gets wide publicity in the newspapers. Kane (Jeremy Slate) shows up and begins working at Shiloh where he reveals to Randy that he is not only his long lost older brother but also that he is evading the law on charges he claims were false. The publicity also attracts a US Treasury agent, Duggan (Dick Foran), as the coins seem to be a small part of missing Confederate assets. A treasure hunt ensues in which Randy is ultimately forced to decide on his brother's fate.

Millionaire Paul Leland (Linden Chiles) treats everyone like dirt and ends up in the desert after getting shoved off a train by a disgruntled employee. He is found and taken on by the Virginian and Shiloh crew who are driving a herd of cattle to Seattle. Leland has several run-ins with the crew as well as former employees met along the way but in the end becomes a better man for his tribulations.

Trouble comes to Medicine Bow in the form of a number of settlers who camp on land they say has been promised to them but that Judge Garth and others traditionally have leased from the government for grazing cattle. The land settlement has been promoted by Congressman Matt Cosgrove (James Dunn) who had read law with the Judge 30 years before. The Judge knows the soil is no good for farming but is told government reports support the reverse. While the Judge and Cosgrove are at odds, Cosgrove's nephew, Army Lt. David O'Mara (Martin West), and Betsy Garth find each other more to their liking.

An older man, Murdoch (Franchot Tone), and his young grandson Willie (Billy Mumy), arrive at Medicine Bow on foot. Much to the Virginian's chagrin, Trampas feels sorry for Murdoch and offers him a job at Shiloh. Murdoch's ineptitude causes several problems, and he begins to resent his grandson's admiration for top cowboy Trampas. In the end, however, Murdoch more than proves his worth and gains the admiration of all, including his grandson.