The wife of a famous baseball player is brutally killed in her home and her philandering husband is charged with the crime. The jury on the case, as well as the accused and his defense, visits the house to examine the crime scene, but when the lights are dimmed, as they were when the victim was killed, and then turned back on, the ball player is found dead with a meat clever in his back and a note that reads "guilty." Now, as Horatio and the team find that the husband's defense team tampered with the crime scene, they must examine the evidence to find the couple's killers. Meanwhile, Horatio gets a call from Yelina, who tells him that her son, Ray, Jr., is missing.

During a routine traffic stop, a police officer is murdered and now Horatio and the team must track down the killer. The only witness to the murder is a civilian who was doing a ride-along with the officer, but who is now missing. Roadside evidence leads the CSIs to Missy Marshall, a teenage girl who was with the killer at the time of the murder. However, as the team searches for the civilian and the killer, they find that Missy may have been more involved with killer and the shooting than she is admitting. Meanwhile, Horatio and Rebecca's relationship is in jeopardy when they don't see eye to eye on a case.

Evidence leads the CSIs to believe that someone started a massive fire in the Everglades in order to cover up a murder. Local prisoners are brought in as free manpower to help fight the wild fire in the Everglades. However, when a serial killer escapes the scene, Horatio must track him down before he strikes again. Meanwhile, the body of a local college student is found shot to death and the fire has destroyed the crime scene and the evidence. However, when the team uncovers an illegal moonshine operation and homemade ammunitions in the vicinity, the CSIs uncover the real reason why the boy was out there.

Horatio's life is changed forever when he learns important information about his deceased brother. Horatio must now figure out what to do with this information about his brother, Raymond, and whether or not to tell Yelina. Meanwhile, the team investigates when a woman is murdered at a crowded bar after she's approached by a female employee of a service that helps men pick-up women. Also, when Delko loses his badge after some reckless behavior and it's used in a crime, he must locate the badge before it's used again.

An axe murderer on death row is given a stay of execution just moments before it was to take place, on the grounds that there may have been tainted DNA analysis used at his trial. Now, without using any DNA evidence, the team must race against time and reexamine all of the physical evidence in the case, just as they get a call on a new double murder that looks suspiciously like the case they're working on.

The team must find the connection between the two crime scenes; the killing of a drug dealer and the rape of a well-off family member.

The search of a missing 17-year-old girl, whose trip to Miami turns into an ongoing nightmare.

When Horatio's nemesis, Walter Resden targets someone close to him, Horatio tries to stop him. Also Eric Delko's relationship with Natalia is tested when she thinks she may be pregnant.

After a man is found not guilty of his wife's murder the CSI team finds evidence to the contrary.