More than half of the members of the Royal Order of Religious Knights were massacred due to the last attack on them that was conducted by the Valentine Brothers. Sir Integra Hellsing, Arucard, and Seras must now hunt down the perpetrators. The suspects include the Vatican's Iscariot Organization. Arucard must do battle once again with the notorious Alexander Anderson.

Almost commiting suicide, Integra is being operated on to save her life. While the operation is going on, we see the history of Integra's ascension to power. We learn that her uncle tried to take her father's place as head of the Hellsing organization, and in attempt to assume power, tried to kill off Integra while Walter was away (presumably on duty). Integra recalls her father's words of her going to a prison cell on the bottom floor if she ever found herself in a moment of insurmountable peril. There she finds Arucard, while at the same time found and attacked by her uncle and associates. After being shot in the shoulder, her blood awakens Arucard, who quickly takes care of her uncle's goons, and recognizes her as her father's successor. As her first encounter with Arucard concludes, she awakens from her state to find that the operation was a success... Walter has already set up her afternoon tea for her.