Leo discovers three teen super-humans Adam, Bree and Chase, living in a secret underground lab in the high-tech home he shares with his inventor stepdad, Donald Davenport, his mom, Tasha, and Eddy, the "smart house" computer. Leo takes the trio to school, but when they are unable to contain their bionic abilities on campus, Davenport sends them away to a remote secret lab.

On the Lab Rats' first day of school, all goes awry when Chase is confronted by a football player and his "Commando App" is triggered, transforming him into Spike, an unruly drill sergeant. When Spike defies the school principal, she pits the Lab Rats up against the football team to battle it out for the coveted cafeteria table

When Chase refuses to admit that Adam has good ideas Adam decides to run against him for student of the semester. Meanwhile, Leo takes advantage of Bree's super speed and starts using her as his personal taxi.

Leo & Adam team up for the school fitness challenge expecting Adam's bionics to be his secret weapon; the plan backfires when they end up going head-to-head in the finals. Meanwhile, Bree and Chase use their bionic abilities against one another.

Adam, Bree and Chase switch their bionics but things end up not the way they think when they can't handle it anymore.

Adam, Bree, and Chase are caught on Principal Perry's surveillance camera using bionics.

Leo turns to Bree for help after he mistakenly unleashes a swarm of robotic flies on the school. Adam and Chase turn their class assignment into a destructive competition.

Leo finally goes a mission as he helps Davenport and Eddy attempt to stop an underground gas leak. Adam, Bree and Chase struggle to escape from Principal Perry’s detention lockdown.

Adam's confidence plummets after Chase pranks him, so Chase tries to make things right by creating a fake rescue scenario, but his plan backfires and puts him in real danger.

When Chase is not pictured on the yearbook, he enters a talent show to show everyone he's popular by using his bionics to move a chair with his mind. But Leo messes with Chase's bionics and he was mad so he got revenge by going into commando app and then he messes with the kids in the school which causes Davenport to turn off his bionics until he learns his lesson.

The Lab Rats accidentally damage Davenport's one million dollar painting.

Adam gets a truck. Leo tries to get her mother out of being PTA teacher.

Davenport races Joey Logano.

In an effort to get out of training duties, Adam enlists Leo's help to create a duplicate Adam using Davenport's experimental cloning device. However, Adam's clone uses the same device to create another duplicate to avoid going to school and the clones he creates repeat the same process resulting in a mob of ever duplicating Adams.

When the school mascot's Dingo costume is stolen by the rival team, Adam seeks revenge by taking their mascot, a llama. When Chase and Adam try to return the llama they discover that the animal has swallowed hundreds of Davenport's mechanical nanobots causing it to shoot acidic bionic spit.

When Leo discovers that his nemesis Trent is rattled about a ghostly legend at Mission Creek High, he comes up with a plan for Chase and Adam to use their bionics to stage a haunting at the school.

Chase inadvertently causes Adam to shrink down to two inches.

The Lab Rats go on a rescue mission to save Dr. Evans and travel to Davenport's secret facility in the frozen tundra.

Leo and Chase use Davenport's technology to get back at Trent on the day of a big test; Bree gets exposed to a hair growth cream with bad results.

The Lab Rats are forced to expose their bionic secret in order to save Principal Perry.

Leo and Donald escape the elevator. Principal Perry lets them stay at the school temporarily in order to get her money from Donald quicker. The Lab Rats stow away on a cargo ship to hide from the FBI and must choose to either keep quiet or help stop a submarine that will crash into the ship and risk being caught. The Lab Rats decide to stop the submarine, with Chase activating his GPS, revealing the Lab Rats' location. Douglas ends up capturing them, disguised as Donald. Douglas reveals that his new bionic partner is a billionaire named Victor Krane, who wants to destroy the Lab Rats permanently. When Donald calls the Lab Rats and tells them that Principal Perry kept their secret, they return home, only for them to get crazy as a result of the Triton App and attack the family, Leo manages to disable Chase's Triton App when he talks to him and Chase manages to disable Adam and Bree's apps. The Lab Rats and the family later see the demolition that happened to the lab and are upset.

Leo and Donald escape the elevator. Principal Perry lets them stay at the school temporarily in order to get her money from Donald quicker. The Lab Rats stow away on a cargo ship to hide from the FBI and must choose to either keep quiet or help stop a submarine that will crash into the ship and risk being caught. The Lab Rats decide to stop the submarine, with Chase activating his GPS, revealing the Lab Rats' location. Douglas ends up capturing them, disguised as Donald. Douglas reveals that his new bionic partner is a billionaire named Victor Krane, who wants to destroy the Lab Rats permanently. When Donald calls the Lab Rats and tells them that Principal Perry kept their secret, they return home, only for them to get crazy as a result of the Triton App and attack the family, Leo manages to disable Chase's Triton App when he talks to him and Chase manages to disable Adam and Bree's apps. The Lab Rats and the family later see the demolition that happened to the lab and are upset.

Since the lab is destroyed, the Lab Rats go without their capsules. This causes glitches in their bionics. In order to prevent this, Donald pulls off a huge stunt in a fiberglass jet wing he invented. Things go wrong when the jet wing runs out of fuel. Meanwhile, knowing the Lab Rats' bionic secret, Principal Perry uses Adam and Bree as her bionic servants for the day.

Since Principal Perry knows that the Lab Rats are bionic, she wants to get in the action and go on a mission with them. When they stage a fake mission and take her down to the newly built lab, things don't go as planned, and they have to save Mission Creek High from a speeding rocket. Meanwhile, Janelle goes on a date with Leo, but is beginning to see him as a jinx because she always gets hurt when he is around.

Chase loses the phone with a bowling video of him and Adam doing bionic bowling stunts at Tech Town while working. Leo and Bree try to get Principal Perry to spend her money when she retires after Donald gives her money and Trent becomes the principal and makes Leo's life miserable. It works in the end, but Principal Perry is broke and Scott fires Caitlin after she destroys an ePhone 7, the one that had Adam and Chase's bowling stunts on it, while giving them to the customers as revenge. Leo makes Trent wear a different uniform, but to Bree and Leo's shock, he loves it, much to their dismay.

During a lunar eclipse, Principal Perry arrives at the house to inform everyone that aliens are on the planet, much to the cha Bree, Chase, and Donald's chagrin, but Adam and Leo's shock. She insists that Chase's upset stomach is due to an alien growing inside him and chases him. However, they notice green goop leaking out of Principal Perry's ears and mouth. Donald then admits that she's an alien. Principal Perry chases everyone, while Leo records the whole thing, and eventually tires herself out. Leo accidentally wakes her and she runs off with him to the school. Adam's heat vision destroys the alien pods Principal Perry laid and Leo is freed. The mother ship beams Principal Perry away just as Donald arrives. Thinking that they will miss Principal Perry, they are sadly mistaken when the mother ship beams alien-less Principal Perry back down. It is then revealed the whole thing was Leo's entrant in a contest.

Leo's scared by a shark movie, so Adam and Chase use one of Donald's sharks to scare him, but it goes haywire and Adam, Chase, Leo, and Janelle end up stuck in the house. In the end, Leo turns the sharks off. Meanwhile, Principal Perry enlists Bree and Donald's help to get into a country club, but when she lies that Donald is her husband and Bree is her daughter and they have pottery around the world, Bree must use super speed to go places without the country club owner knowing about her bionics. Eventually, Principal Perry doesn't get in after the manager figures out the lies, but she decides to spend the summer with the Davenports.

When the Lab Rats are on a mission, it is revealed that a girl was secretly recording them behind a bush. The next day, the Lab Rats go to school and everyone is looking at a viral video called The World's First Bionic Humans. They go back home to tell Donald, but he says he already knows. Meanwhile, Chase is confused that he didn't notice the girl who filmed them behind the bush, even though he thermal scanned the whole place. He then looks at a chip log and finds out that the girl was in the exact spot he scanned. Chase then goes to tell Donald, but a swarm of government agents surrounds them. Leo and Douglas are still in the lab, but they use a trap door to escape and go to school to stay safe. It is then revealed that the mission the Lab Rats were on was a setup. Krane then shows up with the girl who filmed the Lab Rats, whose name is revealed to be S-1. It is also revealed that she is bionic. Krane electrocutes Principal Perry and Douglas while S-1 crushes Leo's arm. Later, Douglas and Principal Perry help Leo to get his arm fixed and take him to the hospital. Tasha later shows up, only to realize that Leo is gone. While the Lab Rats are saying their goodbyes, Special Agent Graham tells them it's time to leave, but Adam refuses. Agent Graham orders his agents to take them down, but Adam uses his wave blast ability to knock them down. When they run to the hospital, Principal Perry tells them Krane is responsible for exposing their bionics. Meanwhile, Leo is in Douglas and Krane's old warehouse and Douglas tells him to take his bandage off and pretend to throw a ball. He then realizes that Douglas upgraded his right arm with bionics. Krane then shows up and knocks down Leo and Douglas. The Lab Rats then show up with Donald, as Krane uses a powerful bionic ability that knocks out Leo, Donald, and the Lab Rats. Krane then paralyzes Donald with his choking ability. Bree battles S-1 while Adam and Chase battle Krane, but he easily takes them down with his newly upgraded Triton App that he uses on S-1. Leo then uses his bionic arm to take down Krane. As Agent Graham arrives, Douglas reveals that Krane and S-1 are the bionic villains. Agent Graham is then saved by Bree as Krane tries to attack him and he takes Krane and S-1 into custody and locks them in a prison with a bionic signal interrupter, preventing them from using their bionics to get out. Later, the Lab Rats are at a press conference and reveal that they are bionic superhumans. While watching it, Principal Perry makes new friends with the Lab Rats. In the end, Agent Graham tells Donald he's now in charge of the Lab Rats. It is also revealed that Krane has an army of bionic soldiers, which he will use to destroy the Lab Rats.

When the Lab Rats are on a mission, it is revealed that a girl was secretly recording them behind a bush. The next day, the Lab Rats go to school and everyone is looking at a viral video called The World's First Bionic Humans. They go back home to tell Donald, but he says he already knows. Meanwhile, Chase is confused that he didn't notice the girl who filmed them behind the bush, even though he thermal scanned the whole place. He then looks at a chip log and finds out that the girl was in the exact spot he scanned. Chase then goes to tell Donald, but a swarm of government agents surrounds them. Leo and Douglas are still in the lab, but they use a trap door to escape and go to school to stay safe. It is then revealed that the mission the Lab Rats were on was a setup. Krane then shows up with the girl who filmed the Lab Rats, whose name is revealed to be S-1. It is also revealed that she is bionic. Krane electrocutes Principal Perry and Douglas while S-1 crushes Leo's arm. Later, Douglas and Principal Perry help Leo to get his arm fixed and take him to the hospital. Tasha later shows up, only to realize that Leo is gone. While the Lab Rats are saying their goodbyes, Special Agent Graham tells them it's time to leave, but Adam refuses. Agent Graham orders his agents to take them down, but Adam uses his wave blast ability to knock them down. When they run to the hospital, Principal Perry tells them Krane is responsible for exposing their bionics. Meanwhile, Leo is in Douglas and Krane's old warehouse and Douglas tells him to take his bandage off and pretend to throw a ball. He then realizes that Douglas upgraded his right arm with bionics. Krane then shows up and knocks down Leo and Douglas. The Lab Rats then show up with Donald, as Krane uses a powerful bionic ability that knocks out Leo, Donald, and the Lab Rats. Krane then paralyzes Donald with his choking ability. Bree battles S-1 while Adam and Chase battle Krane, but he easily takes them down with his newly upgraded Triton App that he uses on S-1. Leo then uses his bionic arm to take down Krane. As Agent Graham arrives, Douglas reveals that Krane and S-1 are the bionic villains. Agent Graham is then saved by Bree as Krane tries to attack him and he takes Krane and S-1 into custody and locks them in a prison with a bionic signal interrupter, preventing them from using their bionics to get out. Later, the Lab Rats are at a press conference and reveal that they are bionic superhumans. While watching it, Principal Perry makes new friends with the Lab Rats. In the end, Agent Graham tells Donald he's now in charge of the Lab Rats. It is also revealed that Krane has an army of bionic soldiers, which he will use to destroy the Lab Rats.

Donald and the Lab Rats attend a fan convention about the movie, Alien Gladiators. The Lab Rats queue up to meet Andre Ethier, but Caitlin comes and makes them let her cut in line in front of them, getting the lost spot and meeting Andre Ethier. They fight with Caitlin and are thrown in a security holding area with a fanboy. Meanwhile, Leo and Donald are taking part in the Fire Staff Competition to get the walk-in role in the next Alien Gladiators movie.

Chase feels that it is unfair to be picked on by Adam every time and tells Donald. Douglas overhears and unlocks a new ability for Chase, upsetting the balance in the house. Douglas tries to fix things by giving Adam a new ability as well, but Adam and Chase end up fighting with one another. Meanwhile, Bree tells Leo about a contest at Tech Town as they are about to hit their one-millionth customer and Leo competes to ride on the first anti-gravity roller coaster and meet the owner of Tech Town. Principal Perry tries to compete to crush Leo's dream. In the end, Chase and Adam have a death battle, but eventually forgive each other and find out that Douglas gave Bree the ability to go invisible, scaring everyone in the lab.

Chase gets a girl interested in him, but Adam gets jealous and tries to ruin their date by having Chase turn back into Spike. Meanwhile, Leo and Bree suspect that Principal Perry has murdered a cook from the cafeteria and Donald. They then find out it was about a hamburger truck and they misunderstood everything.

Bree wants to get revenge on Adam for embarrassing her in front of her boyfriend, Jake. Working with Chase, she puts on a cyber cloak that makes her look and sound exactly like Principal Perry and embarrasses Adam in front of the whole school. However, when she shows Adam the cyber cloak right before the dance that night, it glitches and won't come off. Bree then makes Chase wear a cyber cloak of herself and go to the dance with Jake. While that's going on, Leo and Douglas snoop in Donald's briefcase and compete for what they think is to be his new vice-president, only to find out it was for the Davenport Industries softball team. Donald then finds out that he had two cyber cloaks missing and deactivates them. This embarrasses Chase and gets Bree into trouble with Jake. She then thinks she's patched things up with Jake, but it's really Principal Perry wearing a cyber cloak.

The Lab Rats plan to gain good publicity by having cameramen film them during a mission, but it turns into a disaster when they fight for screen time. After the mission, the president orders the team to stay away from the public in order to let him settle things down. However, it goes wrong when Leo and Principal Perry are holding back angry protesters who want the Lab Rats gone, and Leo accidentally uses his bionic arm. As the protesters become even more enraged, they try to storm the house; Adam panics and uses his bionic super breath to blow everyone off the premises. However, Chase criticizes Adam's actions and the Lab Rats start fighting. They eventually decide to break the team. Three weeks later, the Lab Rats refuse to talk to each other, but they are forced into action when Krane plans to use his Triton App on everyone in the world. A large battle ensues, pitting Leo, Donald, Douglas, and the Lab Rats against Krane and his bionic army. Most of the soldiers attack Adam and Bree, who manage to take them out. Chase has a one-on-one battle with S-3 and Leo has one-on-one battle against S-1. Donald and Douglas attack the soldiers surrounding the satellite, but Krane keeps them from getting too close. Eventually, the Lab Rats and the rest of the team manage to hold back many of the soldiers, but they are running out of time to stop Krane. Douglas then reveals that the Lab Rats can fuse their abilities to stop Krane. They succeed, blasting Krane into the sky and breaking his control over the soldiers. However, their victory is cut short when they find out that Donald is in serious condition after the battle. Later they find out that after the surgery, Donald is still in a very delicate condition. Leo is made an official member of the Lab Rats and gets an upgrade to his bionic arm.

When Leo is chosen for a mission over Spin, Spin recruits Bob to join him on an unauthorized mission to prove themselves. They both end up stuck in quicksand and call Leo to save them. After Leo rescues them, Donald decides to move Leo up a color. Meanwhile, Principal Perry returns saying that she wants a job as head of security at the academy. After countless attempts, Donald agrees, leaving Leo in a particular despair. Meanwhile, Sebastian tries to help Chase get revenge on Adam with a virtual reality machine. After that, Chase tells him about his creator and how they took him out. Later, Sebastian injures Donald with the machine used to prank Adam. When the Lab Rats take Donald to the medic, Sebastian turns evil and says that if they took away his father, then he is going to take away theirs.

Sebastian has secretly formed an evil rebellion to get revenge on the Lab Rats for destroying his father. Meanwhile, Leo is trapped with the other bionic people who also want revenge, but Leo manages to convince Spin and Bob to help him, and, in the end, Sebastian is defeated, his rebellion is brought down, and he and his comrades are taken to a facility. Meanwhile, Donald successfully convinces the others that he has the best interest for them at heart while Adam still blames Chase for everything that happened after the rebellion is over.

Sebastian has secretly formed an evil rebellion to get revenge on the Lab Rats for destroying his father. Meanwhile, Leo is trapped with the other bionic people who also want revenge, but Leo manages to convince Spin and Bob to help him, and, in the end, Sebastian is defeated, his rebellion is brought down, and he and his comrades are taken to a facility. Meanwhile, Donald successfully convinces the others that he has the best interest for them at heart while Adam still blames Chase for everything that happened after the rebellion is over.

When suspicious events happen overnight on the bionic island, everyone is convinced a new threat has emerged. However, this threat is revealed to be Leo, when his bionics glitch because Douglas never gave Leo a capsule.

Donald's top scientist, Dr. Ryan, arrives to test Chase's invention. The two of them dislike each other and don't get along. When Donald puts Dr. Ryan in charge of the space elevator, Chase immediately gets jealous. Donald then makes Chase Dr. Ryan's assistant. Dr. Ryan puts Chase in charge of screwing in the lug nuts, but Chase refuses, and Dr. Ryan builds the space elevator without Chase. The day of the launch, Chase performs a bionic scan of the elevator and finds a major braking flaw on the elevator.

Douglas creates a device that makes any bionic human as smart as Chase. After Douglas tests it on Bob, Bob starts spending time with Chase. This leaves Adam in envy. Meanwhile, Perry made the island officially her own, which makes Bree and Leo angry at her.

Donald gives Eddy a human body and replaces his disrespectful personality to make him nicer. Perry restores his old personality, and he attempts to harm the students.

Leo just finishes his movie marathon while everyone else is frustrated because they have nothing to do on Halloween. Bob intentionally stays behind while the other students are away with Davenport to a museum. Perry says she's going to another island across from theirs, but Douglas says no because it's deserted and has been uninhabited for years. When Douglas leaves, the group decides to go anyway.

Tasha arrives on the Bionic Island informing everyone that she has tickets to a big football game of the Pioneers (mascot of a college that she went to) vs Vikings (mascot of a college that Perry went to) and Perry arrives stating that she has been trying to score tickets for weeks but it was sold out. When she asks Tasha if she has any tickets, Tasha lies saying that she hasn't. When they are about to enter the game, Perry eventually finds out the truth, she becomes furious and destroys the tickets which causes Leo, Donald, and Tasha to try to get new tickets competing against her in The Ultimate Tailgate Challenge, hosted by NFL former linebacker Willie McGinest. Meanwhile, Bree attempts to achieve her college experience by fitting in with a couple of college students (who are football fans) and attempting to use her bionics to impress them.

In the middle of a massive heat wave, the whole family is going to space to see Donald's space colony, called "Davenportia". Adam annoys Chase while Chase tries to give his safety instructions and accidentally blasts them into space. After leaving the solar system, they initiate hyper-speed and land on the planet Lithios. In the dome, it's discovered that Perry sneaked her way in with the rest of the colonists.

Donald and Douglas reveal that they have built an upgrade for all the students bionics, except for Leo's. But the upgrade doesn't work on Adam, Bree, and Chase because their systems are older. Adam, Bree, and Chase are therefore left at their level, while all the students are much more powerful. Meanwhile, Daniel is brought to the Academy because he had been using his bionics in the outside world. Douglas decides to enroll Daniel at the Academy, and he makes Leo his mentor. Leo steadily tries to teach Daniel, but Daniel proves to be unteachable, so Leo gives him the upgrade as well. The students steadily start to vanish, including Daniel. Giselle Vickers, Douglas' old girlfriend, is revealed to have captured them. Daniel sends a video message to the Island, requesting help.

Giselle reveals she dug up Marcus from Douglas' old lair and rebuilt him, piece-by-piece. Marcus wants revenge on Douglas for leaving him to die. Everyone rushes Giselle and Marcus, but Marcus knocks everyone unconscious. Donald and Douglas are tied together in chairs, while Adam, Bree, Chase, and Leo are put in the chamber with the students, while Daniel is pulled out.