The king of Austrian's grandchildren board a train carrying highly valued artworks, and to get to her target Fujiko impersonates herself as Maria, their tutor. While on board, she runs into a mysterious samurai from the East - Goemon Ishikawa.

Lupin and Jigen find themselves looking after a girl who was used as a living piece of art, whilst evading Fujiko, who desperately wants to kill her for personal reasons.

Fujiko enters a chamber where Count Luis Yu Almeida is sitting and waiting for her, however Lupin interferes from a communications room and being gunned by Jigen, who inhaled the Fraulein Eule Cult drug, which makes Lupin flee. Fujiko falls into another chamber where message appears in blocks and Lupin assists Fujiko to reach again the top. In a special room, Lupin explains the real events that occurred in the tragedy of Eulenspiegel and the process of brutally inducing mind control to little girls. Also in the room lies Dr. Kaiser's daughter Aisha who has been long time experimented for the sake of Count Almeida and now frail and weakened. Fujiko reveals that she entered the manor as a maid and intends to steal the Fraulein Eule cult drug, however she's been cornered by Almeida's men and experimented her as well. Her drive to steal and indulge in sex made Aisha somewhat happy.