Overwhelmed by recent events, Ryuko demands answers from her homeroom teacher, Aikuro Mikisugi. Meanwhile, Uzu Sanageyama, Honnouji Academy’s Athletic Committee Chair and on the Elite Four, makes a request of his own… to challenge Ryuko to a duel!

The final round of Honnouji Academy’s Naturals Election is about to begin and the Elite Four all want to be the first to fight Ryuko. Her homeroom teacher, Aikuro Mikisugi, is concerned if she’ll be able to make it through one fight, let alone all four!

The second battle against the Elite Four begins! How will Ryuko defeat Houka Inumuta, who is using the data he’s collected to predict her every move? Watching the action, Mako and her family deal with a few surprises of their own!

Ryuko's rampage continues, and thanks to Ragyo and Nui she has an unexpected new ally. With Satsuki as the target, will the Elite Four and Nudist Beach be able to withstand the onslaught?

The ongoing battle presses towards Honnouji Academy, but Ragyo and the Original Life Fiber stand in the way. Ryuko and Satsuki set off to face them, leaving Nudist Beach's defense to... Mako?! Meanwhile, a crazed Nui continues working feverishly...

The final confrontation is at hand! Will Ragyo and Nui bring on the end of the world, or will Ryuko and the others be able to stop them?