In the same frame-story format, Hader and Samberg apologize to Lorne Michaels for the original Laser Cats! claiming to understand where they went wrong: not enough politics. They then introduce Laser Cats! 2, based on the same premise, now set in the Iraq War which has gone nuclear and caused the feline mutations. This time, Dr. Scientist (Jake Gyllenhaal) has stolen the cure that turns Laser Cats back into regular cats. Jorma Taccone appears in the opening credits for Laser Cats! 2 (Which is the same as Laser Cats!), and Fred Armisen and Amy Poehler are the scientists who created the cure.

A corporate executive (Rainn Wilson) leads a meeting to take ideas on how to save his failing company, and hears suggestions from an increasingly bizarre set of employees, including a gigantic turkey sub and Arcade Fire. Upon reaching the end of the meeting, Wilson receives a phone call, seemingly informing him that the office building is about to be blown up. Then, the office building explodes, killing all of them inside.

In a spoof of The O.C. episode "The Dearly Beloved", a man (Hader) writes a letter to his sister, he is shot by his friend (Samberg), leading to a series of overly dramatic, slow-motion shootings set to "Hide and Seek" by Imogen Heap, including their roommate (Shia LaBeouf), the sister (Wiig), and two police officers (Sudeikis, Armisen).

Neil Patrick Harris and the entire SNL cast perform the theme to Doogie Howser, M.D.. Harris wears a tuxedo while everyone else is dressed as Doogie Howser in blonde wigs, lab coats with a picture ID of Doogie on them, dress shirts, colorful ties, acid washed jeans, and Nike hi-tops (Armisen and Samberg wear green sunglasses as well). Harris plays keyboard; Wilson, Elliott, and Michaela Watkins play violin; Moynihan plays tuba; Thompson plays harp; Hader plays saxophone; Hammond plays trumpet; Wiig plays electric guitar; Sudeikis plays double bass; Forte plays drum kit; Armisen plays synthesizer; and Samberg plays an Akai MPC 2000XL drum machine. At the end of the performance, Harris sheds a single tear.

Samberg and Hader are at a party where everyone and everything is a literal representation of a slang term (i.e., a "serial rapist" (Forte) is shown having sex with a box of cereal, a group of "motherfuckers" are men with their mothers, a group of "sons of bitches" are a litter of puppies, a "numbnuts" is a man (John Lutz) shown sitting on a block of ice with his pants around his ankles, a group of "jokers" are dressed as different incarnations of The Joker from The Dark Knight, a knucklehead is a hand with plastic eyes glued on it, etc.). When Samberg and Hader discover "two douchebags", however, it's revealed that they're looking at themselves in a mirror.

During a performance review conducted by episode host Seth Rogen, Samberg raps about his typical day as the boss of a large company. The first few activities he names are what one might expect, such as approving memos and micromanaging his staff (John Mulaney). His actions become increasingly erratic, however, after a subordinate (Kristin Wiig) rejects his advances and he is consequently denied a promotion. The boss's description ends with him turning into a jet, crashing into the sun, and dying. Rogen leaves the performance review in apparent disgust as Samberg repeatedly reminds him, "I'm the boss."

The fifth single from The Lonely Island's debut album, Incredibad, Rogen's voice replaces Schaffer's as the company evaluator, though Schaffer does appear in the short as a hooded gun dealer. Taccone also makes a cameo appearance as the man who hands Samberg a notice about a harassment lawsuit filed by Wiig.

An unemployed cocaine addict named Dennis (Samberg) goes on drug binge while singing an elaborately choreographed Disneyesque musical-theater number about how it is going to be a great day. The more cocaine he snorts, the faster he sings, and the more disjointed and surreal the song becomes. Eventually the whole experience is revealed to be a vivid, cocaine-induced hallucination. 9th music video on Lonely Island's album, Turtleneck & Chain.

A parody of the movie Weekend at Bernie's, in which two men (Hader and Samberg) find the corpse of their boss (played by episode host Robert De Niro) and attempt to make him look alive during a party at his beach house. They are arrested and put on trial, and proven innocent by Mr. Bernard's video will asking for the exact actions that the two had taken.

Andy Samberg and Adam Sandler sing about times when people broke character on the show. Chris Parnell and Bill Hader also appear.

This short was written and produced for the SNL 40th Anniversary Special.