When Eliza, Darwin and Donnie are flung from a Matadi-bound barge and stranded on the banks of the Congo river, they must traverse the cliffs and crags of mountainous Zaire in order to catch up with her family.

As animals flee their homes and run for their lives, Eliza discovers that Kip and Biederman are destroying part of the rainforest. In an all-out war against the evil duo, Eliza must convince the animals that with everybody's help, they can stop the destruction and save the forest.

When Eliza gets a hunch that foul play has led to Darwin's disappearance, she sets out to rescue her long lost companion. To her dismay, she finds Darwin has become one of the most popular acts at an Indian palace where a young prince lives. It's up to her to prove to Darwin that friendship runs deeper than the glamour and glitz of showbiz.