Everything is normal as the four main characters attend a normal day of high school. They discuss different foods and how they eat them. Later on, Kagami gets sick and all the characters go over to make sure she is okay.

Throughout Golden Week, Tsukasa and Kagami have been playing together. However, Tsukasa does not know that her sister has actually been waking up earlier and going to bed later than her in order to study and finish homework. After the break, Konata does an all-nighter and manages to obtain an above-average score despite doing no studying during the short vacation. Later, Konata decides to get a job at a cosplay café in order to fund her otaku desires.

It is summertime for the girls; that means one thing: comfortable summer uniforms! Tsukasa and Kagami are fraternal twins, but Konata shows them the similar and different characteristics they share. Konata, Kagami, and Tsukasa each try to put their hair into a ponytail, just to see who looks best. Unfortunately, Kagami is the only one who cannot pull it off.

It is time for tests! Unfortunately, Konata cannot seem to concentrate on studying and instead reverts to slacking off and playing games. She tries to ask Miyuki about good study habits, but in the end she does bad on her tests. Tsukasa got help from her sister and this time got better scores, but Kagami's scores were still higher despite her own scores going down somewhat. Later, Konata and the Hiiragi twins go to a cake café but end up taking too much and must finish it all or pay an extra price.

Winter break is over, and so it's back to school where the girls have conversations about what they did, ate, and dreamt about during their winter vacation.

It's now time for Valentine's Day, which means that the girls will give chocolates to others, but in the case of Konata, she'll give her chocolate to a character online.

Lster, Konata decides to shop at an anime store she previously went to but realized that the item she wanted was at another store. The employees of the anime shop are determined to get Konata (a.k.a. Legendary Girl A) to buy something from their store this time around.

This week on Lucky Channel, Akira and Shiraishi announce the results of the Lucky Star figure poll, telling which characters will get figures made out of them. However, Akira gets really mad when she learns that Shiraishi will also have a figure made.

It's Konata's birthday, and her family sets up a surprise party for her when she gets home from work. All goes according to plan until Konata calls and says that her coworkers are throwing her a party and that she'll be coming home late.

The girls discuss what their goals in life are.

Kagami has a bad day when she gets bothered by little annoyances and ends up going to a store to buy a drink but forgets her wallet.

Things get worse for Kagami when her older sister Matsuri scolds her for neglecting to buy groceries for dinner.

On Lucky Channel, Akira and Shiraishi have a special guest: Meito Anizawa, the manager of the anime store that desperately wants Konata to buy something from his shop. He talks about the three factors that determine an anime's success.

Konata thinks about what animal each of the girls, including herself, would be. She sees herself as a fox, Tsukasa as a dog, Yutaka as a squirrel, and Kagami as a bunny.

Konata and Miyuki discuss things that can improve one's eyesight.

Konata complains about how the recent elections have been screwing up the TV schedule.

Tsukasa tries reading newspapers, but it's not long until she finds them hard to read.

Yutaka and Iwasaki's class plays dodgeball during P.E. Later, they hang out with two of their classmates, Hiyori Tamura and Patricia Martin.

On Lucky Channel, Akira and Shiraishi introduce the newest characters of the Lucky Star cast. However, Akira is mad at the fact that she still hasn't appeared in the actual show yet.

Konata and Yutaka catch a cold, and Yutaka's friends, Minami, Hiyori, and Patricia, come and visit her. They, Konata, and Yutaka discuss younger-sister-moe, Minami's lack of breasts and how Hiyori doesn't want Yutaka to read her doujin manga.

After seeing a TV program about how xylitol chewing gum can eliminate cavities, Miyuki decides to test this out. As a result, one of her fillings comes out and she has to go to the dentist.

Kagami and Misao play a video game together. Although Misao is terrible at playing them, she seems to be having more fun than Kagami who's playing to win.

Patricia tells Kagami what music she's been listening to lately and how much she would like to have a maid.

Miyuki tells her mother about her friends' opinions on Minami, but they seem to contradict with what Miyuki's mom recalls from Minami's previous actions.

On Lucky Channel, a woman named Gotouza disciplines Akira and Shiraishi enough that they'll do one last Lucky Channel segment together.