Two mysterious ninja infiltrate Konoha, and one of them bares the Sharingan. Just what are these two after? Why has Itachi Uchiha returned to Konoha?

Kakashi takes on Itachi, with both of them using the Sharingan. However, Kakashi soon realizes that he stands no chance against the legendary ninja. Itachi reveals that he has not come for his little brother, but instead he's come to do something else...abduct Naruto, and Kyubi as well!

Jiraiya and Naruto begin their search for Tsunade, but are constantly sidetracked by Jiraiya's peeping and hitting on pretty girls. Jiraiya tells Naruto some stories about the 3rd and 4th Hokages. Meanwhile, Sasuke learns that his brother, Itachi, is after Naruto and goes to warn him.

Sasuke remembers the day that Itachi single-handedly wiped out the entire Uchiha clan as he desperately searches for Naruto. However, Itachi and Kisame have already found him.

Jiraiya and Naruto look on as Sasuke takes on Itachi alone. However, when the young ninja is severely outclassed, Jiraiya is forced to step in. Itachi and Kisame try to flee, but Jiraiya isn't about to let them get away so easily.

Now that Sasuke is in safe hands, Naruto and Jiraiya can continue their search for Tsunade. When they arrive at a city with a festival on the way, Jiraiya and Naruto have some fun, at least until Naruto puts a stain on a man's shirt. Jiraiya uses a new technique that easily defeats the man and offers to teach it to Naruto. What is this new technique and can Naruto master it?

Jiraiya gives Naruto the task of rotating the water in a balloon until it pops. Naruto is confident that he will accomplish this stage of the training by night, but soon learns it's not as easy as expected.

After passing stage one of the training, Naruto moves into stage two: power training. Jiraiya realizes that, like him, Naruto has a hard time concentrating, so he paints a spiral in the palm of Naruto's hand to help him to focus. With the aid of the spiral and a quick flashback to Iruka-sensei, Naruto completes stage two. Meanwhile, Tsunade's luck has become uncommonly good, which worries her. After being talked into sightseeing by her assistant, Tsunade meets Orochimaru...and she doesn't look at all pleased.

Naruto continues his training of the Rasengan, having mastered the stages of spin and power. The final stage proves to be the most difficult: control. Meanwhile, Orochimaru and Kabuto accost Tsunade and ask her to restore Orochimaru's arms. If she restores Orochimaru's arms, he promises to use his Rebirth of the Impure World kinjutsu to revive her lost lover and little brother.

Jiraiya and Naruto catch up to Tsunade, whereupon she learns that Konoha wants her to be the 5th Hokage. She refuses the title, angering Naruto, who mistakes her for being a weakling. Naruto learns first-hand how strong she really is. Naruto decides now is the time to try out the Rasengan, only he hasn't perfected it yet.

Naruto makes a bet with Tsunade, that if he can master the Rasengan in one week, she'll give him a necklace belonging the to the First Hokage. However, Shizune reveals some startling information. Everybody, except for Tsunade, who's worn that necklace has died the next day!

While Naruto continues to try and master the third step, Tsunade continues to think about Orochimaru's offer. Jiraiya tries to convince her by taking her out for a drink, only she slipped a paralyzing potion into his drink while he wasn't looking. Will Tsunade agree to Orochimaru's offer? And will Naruto master the Rasengan before the week is up?

Because Orochimaru lied to her about not attacking Konoha, Tsunade begins her onslaught against him and Kabuto. Kabuto eats a food pill and begins to attack Tsunade with his medical techniques but are they enough to defeat her? Will Jiraiya, Shizune and Naruto show up in time?

With Tsunade still paralized with fear and Naruto being a low level ninja, it is up to Shizune and Jiraiya to defeat Kabuto and Orochimaru. Orochimaru and Kabuto summon two giant snakes while Jiraiya summons Gamakichi, who does nothing. Naruto tries to help Jiraya, but he too only summons a small frog that also does nothing. Orochimaru and Kabuto attack and Shizune is easily defeated. Naruto tries Rasengan but fails. Can he master the technique before Kabuto does anything to Tsunade? How far will he go to master it?

Naruto finally lands a Rasengan into Kabuto's stomach, but not before Kabuto assures Naruto's certain death. Can Tsunade save him and protect him from Orochimaru?

With Tsunade in her current conidtion, and Jiraiya still under the effects of the drug, Tsunade and Jiraiya must work together to defeat Orochimaru. And with all three ninja having summoned their signature beasts, it's sure to be one heck of a battle!

The group are on their way back to Konoha when they stop at a town with hotsprings. Unknown to them they are being watched by men looking for Tsunade. Naruto's room gets raided and the necklace he was given goes missing with nothing but a ransom note left behind. What does it all mean? Can Naruto get the necklace back and why are they looking for Tsunade in the first place?

Naruto, Tsunade, and Shizune finally return to Konoha so Tsunade can take over and become Hokage. Konohamaru finds out that Tsunade is the Fifth but refuses to believe it. Naruto then takes Tsunade to the hospital so she can heal Sasuke, Kakashi, and Lee. She heals Sasuke and Kakashi just fine...However, her diagnosis of Rock Lee has heartbreaking consequences for him and his future.

Konohamaru locks himself in the office used by the 3rd to stop them from replacing his grandfather, the previous Hokage. Tsunade spends the day locked out of her office and researches a way to heal Rock Lee so he has a better chance of surviving the operation,

As Lee decides whether or not to go through with the operation, Gai decides to give him some motivation. Meanwhile, Tsunade frantically struggles to increase the operation's success rate.