Gintoki and Shinpachi start re-investigating the Rokkaku incident. Upon learning what really happened from Kamiyama, one of Okita’s subordinates, they leave to save Okita. Meanwhile, Okita is captured by Sotatsu Tendo after falling into his trap involving Kirie. Tendo babbles on about how he returned to seek revenge against Okita, who destroyed the Sokai Faction, and goes on to reveal the truth about Kirie’s father.

The episode begins with a short sequence about Kamui transferring into Yato High School and is told from Abuto's point of view. While the Shinsengumi are out on a mission, Hijikata notices snot coming out of Kondo's nose so he wonders how he could tell him without the others noticing Kondos problem. In the end the snot even takes control over Kondo's body and it comes out that Kondo's true identity is being an Amanto in snot form. As Hijikata destroys it, he realizes it has been all a dream.

It's Christmas in Edo and Kagura writes her father a letter. She is sad that Santa Claus never visited her. Moved, her father Umi Bozu dresses himself up as Santa and hides watching Kagura, Otae, Shinpachi and Hasegawa celebrate Christmas. When he finally jumps out of the bushes to surprise her there is another Santa, a dressed up Gintoki. Both of them think that their counterpart is real. They start punching each other when another three Santas show up (Sachan, Kondo and Kyubei). Kagura decides on a contest to see who's the real Santa.

The preliminary round for the Official Tsu Terakado fan club title is a marathon race. With Takachin in critical condition and Kagura taking the wrong route, team Shinpachi’s chances of winning look slim. But thanks to Gintoki’s quick thinking, Takachin makes an instant (and improved?) recovery and emerges from the hospital to rejoin the race! As the race gradually turns into a battle of deception, Shinpachi runs furiously for the goal. Will he be able to beat the tactical general Tosshi?!

Tama works hard everyday starting early in the morning. Even when Otose tells her to take a break, Tama won’t and says she is happy just being with everyone. But Otose notices that Tama’s appearance has changed into that of an angular polygon from an old video game! While Otose and Gintoki discuss that it must have been brought on by overwork, Tama returns from shopping and has now regressed from polygons to pixels!! Why is she going through these changes? And will she be able to return to normal?!

Gintoki and the gang return to Yoshiwara at Hinowa’s request for the first time in a while. Although Hosen’s death has brought freedom to Yoshiwara, it’s also drawn the renewed interest of those who previously avoided Yoshiwara in fear of Hosen’s power. Yoshiwara is now troubled with an increased crime rate, especially illegal drug dealing. Odd Jobs are asked to capture the man responsible. The man in question is said to go by various names, titles, and even faces. The only clue available is the tattooed spider on his neck.

Gintoki challenges Shinpachi in the ultimate Love Chorus tournament in order to break his illusion of his virtual girlfriend.

Everybody's stranded in the middle of the mountains. To make things worse, the Shogun goes missing.

Jellybeans Vanguard Honda is on his fifth year as a Jump editor. However, he's been assigned to the manga Gintaman, which has driven multiple editors insane. In order to save himself, Honda must find a new talented artist so he can be reassigned. One day, he is visited by an aspiring manga artist by the name of N3016. Is this artist as juvenile as his name suggests?

As Katsura and Kyubei fight for the title of the best transgendered, Shinpachi leaves to find that the Shinsengumi members also suffered a sudden change in their selves; Sogo Okita is the leader from the group and orders him and Toshiro Hijikata, Sagaru Yamazaki to attack the Yorozuya. As the three leave, Hijikata knocks out Yamazaki, as he was faking his change. He and Shinpachi learn that an invasion of parasite aliens is responsible for change to their friends and proceed to destroy them. However, they find problems in the outcome, and both Hijikata and and Shinpachi are knocked out in the process, with Gintoki being actually only person without a parasite.

Kagura's older brother, Kamui, visits Kada's cell where she is being kept after having been captured by the Kiheitai and loses at a game of odds or evens. He is approached by one of the 12 captains who tells him he has the 12 captains support if he chooses to overthrow Admiral Abo. Kamui orders his second to kill the members of the Kiheitai, isolating Takasugi so Kamui can duel him. As the Kiheitai's ship is about to be attacked the Admiral double crosses Kamui and imprisons him out of fear. Takasugi visits Kada's cell and loses at a game of odds or evens. After a brief conversation Takasugi rescues Kamui at his execution, overthrowing Admiral Abo. Kamui becomes Admiral and the episode ends with Takasugi visiting Kada's cell one last time to lose at a game of odds or evens.

The monkey, aka "Jugem Jugem... (abbreviated) Runny Diarrhea," has grown close to Kyubei. However, their time together did not last. The Shogun family ordered that the Yagyu return Runny Diarrhea. The monkey escapes from the Shogun family to see Kyubei again. Kyubei and the other Yagyu members are forced to continue a desperate and fruitless search for Runny Diarrhea.

Gintama meets Sket Dance! But not without trouble...

The Shinsengumi and the Yorozuya attend the funeral of a close udon shop owner. Instead of moving on, however, the soul of the old man is overseeing the ceremony.

The Yorozuya and Katsura follow Elizabeth to stop the Renho from taking over the earth.

Everybody's stranded in the middle of the mountains. To make things worse, the Shogun goes missing.

Jellybeans Vanguard Honda is on his fifth year as a Jump editor. However, he's been assigned to the manga Gintaman, which has driven multiple editors insane. In order to save himself, Honda must find a new talented artist so he can be reassigned. One day, he is visited by an aspiring manga artist by the name of N3016.

A character from 2 years ago is back with a request for Gin. This favor will take the Odd-Job Trio right in the middle of Satan Festival in this very special Christmas episode.

After Gintoki saves Tama from Kintoki, he finds the whole town turned against him after Kintoki frames him for the attack! Now Gintoki must face not only Kintoki, but all his former friends too! However, some of them are beginning to have doubts.

Just when it looks like all is lost, the Shinsengumi and Mimawarigumi arrive to lend their aid to our heroes. Sadasada attempts to escape but Gintoki is hot in pursuit.

Sadaharu, fed up with the lack of food at Odd Jobs, decides to leave to fend for himself. On the way he meets a starving puppy that he can't ignore.

Odd Jobs continue to try and fix the Universal Clock, with little success.

Sakamoto has been kidnapped by the pirates they double-crossed in the past! Now, it's up to Mutsu and Gin to rescue him.

Zenzou and the Iga clan have betrayed the Shogun! Their forces are ambushed from all sides as forces loyal to the shogun desperately fight back.

Having caught Takasugi Shinsfake, the ladies' gang decides to teach him a lesson and try and get him to spill the beans on where Gintoki is. How will Shinsfake survive this?