It is the Taisho Period (i.e. 1912-1926). Tanjiro Kamado is living a modest but blissful life in the mountains with his family. One day, when he returns from selling charcoal in town, he finds the remains of his slaughtered family in pools of blood after a demon attack. Tanjiro rushes down the snowy mountain with the sole survivor, his sister Nezuko, on his back. But on the way, Nezuko suddenly snarls, turning on Tanjiro.

The Demon Slayer Corps... An organization that's been hunting down demons since ancient times. Sakonji Urokodaki starts training Tanjiro for Final Selection, the test for joining the unit. Navigating a mountain riddled with booby traps, dry sword swings, waterfall immersion and breathing techniques... A year later, Urokodaki informs Tanjiro that he has nothing more to teach him, and gives him one last hurdle to overcome in front of a giant boulder.

To pass Final Selection, one must survive for seven days on Mt. Fujikasane where demons captured by the Demon Slayers have been imprisoned. So begins the battle for survival between the young swordsmen and the demons. Armed with the breathing techniques and stances he'd learned from Sakonji Urokodaki, Tanjiro slashes demon after demon. His two years of training have not gone to waste. But just then, a morphed demon appears out of nowhere.

Now split into three, the demons have driven Tanjiro into a corner. It is then that Nezuko attacks. Now that she’s become a demon herself, she’s no longer so weak that she needs protection. His mind made up, Tanjiro follows one of the demons into the swamp now spreading across the ground. Waiting for him there is...

It was two demons named Tamayo and Yushiro who came to Tanjiro’s rescue. Using the “Eyeblind” spell, Tamayo guides Tanjiro and Nezuko to her house. There, through his conversation with Tamayo, Tanjiro learns that there is a way to return demons to human form. Just then, two demons in pursuit of Tanjiro locate the house and unleash a furious assault...

Tanjiro’s crisis continues. He uses multiple forms of the Water Breathing technique to hold out against Yahaba’s final Blood Demon Art, the "Kouketsu Arrow." Meanwhile, Nezuko and Susamaru are still battling it out. Worried about the outcome, Tamayo unleashes her own Blood Demon Art.

Every time Kyogai, the master of house, plays his tsuzumi, the room spins and Tanjiro struggles. Debilitated by the fractured bones he sustained in his previous battle, Tanjiro tries to fire himself up as he desperately fights on, but he is overwhelmed by Kyogai's Blood Demon Art. Will he find a way to slice off Kyogai's head?

Tanjiro and Inosuke battle the Demon Slayers trapped by spider webs. After slashing the threads and destroying the webs, they head deeper into the mountain. The farther they advance through the forest, the thicker the threads become, and those under their control start moving in ways impossible for a human being. Stopping the Demon Slayers without hurting them isn't going to be easy, but then Tanjiro makes a certain move...

Tanjiro and Inosuke face off against the Father Spider Demon of Mt. Natagumo. Tanjiro unleashes an attack, only to get hurled away by the demon's overwhelming power. Landing near a river, he comes upon the young demon Rui torturing the Sister Spider Demon. When Rui calls their bond of terror and hatred "a family’s bond," Tanjiro is enraged. So begins the battle between Rui and Tanjiro.

Seeing Tanjiro try to protect Nezuko even as he collapses triggers memories of Rui’s own past. Once a sickly boy, he became a demon after meeting Muzan Kibutsuji who gave him a share of his blood. But after he turns into a demon, his own parents try to kill him. Ever since then, he has been searching for a family. Seeking a bond that he can never hope to acquire...

The injured Tanjiro, Zenitsu and Inosuke are sent to the home of the Insect Hashira, Shinobu Kocho, for treatment. Two weeks later, Tanjiro and Inosuke start rehabilitation training. But the drills are brutal, and they find themselves broken in spirit. Unaware of the grueling regimen, Zenitsu joins them late and is visibly enthusiastic in front of girls, but...

Nezuko, Zenitsu and Rengoku fight against Enmu to protect the passengers. Meanwhile, Tanjiro and Inosuke discover the location of Enmu's neck. They work in tandem to thwart Enmu's Blood Demon Art and sever Enmu's neck. The Mugen Train writhes in pain and derails. What will be the fate of the 200 or so passengers as well as Tanjiro and his friends?

After arriving at the Entertainment District, Tanjiro and his comrades infiltrate each House where Uzui's wives, Suma, Makio and Hinatsuru, work. While working as apprentices and searching for their whereabouts, Inosuke hears that Makio has been shutting herself away from everyone in her room.

As Tanjiro close in on the demon that has been preying upon the entertainment district. But even as he closes in, the others continue to follow their leads toward their query. Who will find the demon first? And will they be able to survive the confrontation when they do?

Tanjiro and Uzui have been separated by Gyutaro’s Blood Demon Art. As Tanjiro joins the struggling Zenitsu and Inosuke, they manage to decapitate Daki at last thanks to their coordinated barrage of sword strikes. However, when Inosuke tries to run off with Daki’s head, he gets pierced by Gyutaro’s scythe. Uzui has also collapsed, leaving Tanjiro and the others with their backs to the wall.

Tanjiro intervenes during an argument between a pillar and an apprentice blacksmith and learns of the existence of an amazing training method.

Genya aims for the neck of the fifth demon that split off from Hantengu, but the demon's neck is shockingly hard, and his sword snaps in half in his attempt to strike it. His gun is also ineffective and Genya is driven into a corner. Realizing that he is unable to sever the neck by himself, he entrusts defeating the fifth demon, which is believed to be the main body, to Tanjiro. Taking on Genya's will, Tanjiro wields his sword against the fleeing demon...

Armed with his memories, Muichiro feels powerful and invincible, convinced that the demonic fifth moon has no chance against him.