Series Cast 26

1. 

   Josh Keaton

   Peter Parker / Spider Man (voice) (26 Episodes)

2. 

   Lacey Chabert

   Gwen Stacy (voice) (24 Episodes)

3. 

   Joshua LeBar

   Flash Thompson (voice) (20 Episodes), Flash Thompson / Car Thief #1 (voice) (1 Episode)

4. 

   Grey DeLisle

   Sally Avril (voice) (7 Episodes), Betty Brant (voice) (3 Episodes), Sally Avril / Betty Brant (voice) (2 Episodes), Sally Avril / Bette Brant (voice) (1 Episode), Stephanie Briggs (voice) (1 Episode), Sally Avril / Erin (voice) (1 Episode), Sally Avril / Voice of OsCorp (voice) (1 Episode), Sally Avril / Coffee Clerk (voice) (1 Episode), Voice of OsCorp (voice) (1 Episode), Sally Avril / Computer Voice (voice) (1 Episode)

5. 

   Alanna Ubach

   Liz Allan (voice) (17 Episodes), Liz Allan / Trina (voice) (1 Episode), Liz Allan / Helena (voice) (1 Episode)

6. 

   Vanessa Marshall

   Mary Jane Watson (voice) (16 Episodes), Mary Jane Watson / Rosie Thompson (voice) (1 Episode), Mary Jane Watson / Hermia (voice) (1 Episode)

7. 

   James Arnold Taylor

   Harry Osborn (voice) (11 Episodes), Harry Osborn / Frederick Foswell (voice) (4 Episodes), Patch / Homunculi (voice) (1 Episode), Harry Osborn / Alan O'Neil (voice) (1 Episode), Harry Osborn / Patch / Frederick Foswell (voice) (1 Episode)

8. 

   Deborah Strang

   May Parker (voice) (16 Episodes)

9. 

   Clancy Brown

   George Stacy (voice) (9 Episodes), Rhino / George Stacy (voice) (3 Episodes), Alex O'Hirn (voice) (2 Episodes), Alex O'Hirn / Ox (voice) (1 Episode), Alex O'Hirn / Rhino / George Stacy (voice) (1 Episode)

10. 

    Alan Rachins

    Norman Osborn (voice) (15 Episodes)

11. 

    Phil LaMarr

    Rand Robertson (voice) (6 Episodes), Rand Robertson / Robbie Robertson (voice) (4 Episodes), Fancy Dan / Homunculus #1 (voice) (1 Episode), Rand Robertson / Fancy Dan (voice) (1 Episode), Fancy Dan / Ricochet / Rand Robertson (voice) (1 Episode), Robbie Robertson (voice) (1 Episode)

12. 

    Ben Diskin

    Eddie Brock (voice) (8 Episodes), Venom (voice) (3 Episodes), Eddie Brock / Venom (voice) (2 Episodes)

13. 

    Daran Norris

    J. Jonah Jameson (voice) (10 Episodes), J. Jonah Jameson / John Jameson (voice) (2 Episodes), J. Jonah Jameson / John Jameson / Colonel Jupiter (voice) (1 Episode)

14. 

    Kevin Michael Richardson

    Tombstone (voice) (4 Episodes), Principal Davis (voice) (2 Episodes), Tombstone / Principal Davis (voice) (2 Episodes), Big Man / Tombstone / L. Thompson Lincoln (voice) (1 Episode), Coach Smith (voice) (1 Episode), OsCorp Security Guard (voice) (1 Episode), Big Man / Coach Smith / Thug (voice) (1 Episode)

15. 

    Peter MacNicol

    Otto Octavius (voice) (4 Episodes), Doctor Octopus (voice) (3 Episodes), Master Planner (voice) (2 Episodes), Master Planner / Otto Octavius (voice) (1 Episode), Otto Octavius / Doctor Octopus (voice) (1 Episode), Master Planner / Doctor Octopus (voice) (1 Episode)

16. 

    John DiMaggio

    Hammerhead (voice) (5 Episodes), Flint Marko / Hammerhead (voice) (2 Episodes), Sandman / Hammerhead (voice) (2 Episodes), Flint Marko / Sandman / Hammerhead (voice) (1 Episode), Sandman (voice) (1 Episode), Hammerhead / Car Thief #2 (voice) (1 Episode)

17. 

    Dee Bradley Baker

    Curt Connors (voice) (7 Episodes), Curt Connors / Lizard (voice) (1 Episode), Curt Connors / Homunculi (voice) (1 Episode)

18. 

    Cree Summer

    Glory Grant (voice) (5 Episodes), Glory Grant / Cobweb / Homunculus #2 (voice) (1 Episode)

19. 

    Crispin Freeman

    Electro (voice) (3 Episodes), Max Dillon / Electro (voice) (1 Episode), Electro / Clerk (voice) (1 Episode), Thug #1 (voice) (1 Episode)

20. 

    Robert Englund

    Vulture (voice) (3 Episodes), Adrian Toomes / Vulture (voice) (1 Episode)

21. 

    Tricia Helfer

    Black Cat (voice) (2 Episodes), Felicia Hardy / Black Cat (voice) (1 Episode)

22. 

    Miguel Ferrer

    Silvio Manfredi / Silvermane (voice) (1 Episode), Silvermane (voice) (1 Episode)

23. Eric Vesbit

    Kraven (voice) (1 Episode), Sergei Kravinoff / Kraven the Hunter (voice) (1 Episode)

24. 

    Ed Asner

    Ben Parker (voice) (1 Episode)

25. 

    Danny Trejo

    Ox (voice) (1 Episode)

26. 

    Stan Lee

    Stan (voice) (1 Episode)

Series Crew 14