Sasahara Kanji is a college freshman who decides to join a student society to share his hidden thoughts on manga, anime and gaming. As he participates in club activities such as visiting dojin shops and anime festivals he opens his mind and resolves that he will make his way into the otaku world.

Doujin Work follows the life of a young girl named Najimi Osana and her exposure into the doujin world. She was first tempted into becoming a doujin artist after seeing how much one of her friends can make at a convention. Najimi loves to draw, though soon learns contrary to what she expected that this new world is anything but easy. As she attends more conventions and meets more people, Najimi eventually manages to find a group of very interesting friends. These friends already have some experience in the field and help her out along the way so that she can someday make a name for herself creating doujinshi.

A mockumentary featuring an animated tale of an average person who slowly becomes an otaku (obsessive fanatic) and eventually becomes the "Otaking"! Between story segments, live action interviews with fictional die-hard otakus take place.