At the turn of the century, the Angels returned to Earth, seeking to wipe out humanity in an apocalyptic fury. Devastated, mankind's last remnants moved underground to wait for the day when the Angels would come back to finish the job. Fifteen years later, that day has come... but this time, humanity is ready to fight back with terrifying bio-mechanical weapons known as the Evangelions. Watch as Shinji, Rei, Asuka and the rest of the mysterious shadow agency Nerv battle to save earth from total annihilation.

The series is a parody of Neon Genesis Evangelion, in which the entire cast of the original series are now everyday students going to junior high school together at Tokyo-3. The series features three Rei sisters: one is a child younger than the rest based on Rei 1, the second is based on Rei 2 (the Rei seen for most of the normal series), and the third Rei is actually based on the hyperactive and klutzy Alternate Rei (seen in the alternate-reality dream sequence from the final episode of the original series). Further, Evangelion Unit 01 itself is one of their classmates, but this time as a human-sized robot who acts as something of a class bully. Even the Jet Alone robot is re-imagined as a character on the series.