Lois Weber (Pittsburgh, Pennsilvània, 13 de juny de 1879 – Hollywood, Los Angeles, 13 de novembre de 1939) va ser una actriu de cinema mut, guionista, productora, i directora, nord-americana que va ser considerada "la directora dona més important que la indústria del cinema americà ha conegut", i "una de les més importants i prolífiques directores de pel·lícula en l'era del cinema mut".

Lois Weber began film work as an actress, then soon began also writing and directing films. She has been referred to as "the most important female director the American film industry has known". Best known for her hard-hitting films that address social issues, she continued making films into the 1920s until she lost favor with motion picture audiences.