Jennie Eisenhower is the daughter of Julie Nixon and David Eisenhower. Her grandfather was Richard Nixon, the 37th President of the United States, and her great-grandfather was Dwight D. Eisenhower, the 34th President of the United States. She grew up in a suburb of Philadelphia. She is a summa cum laude graduate of Northwestern University in Evanston, Illinois, where she majored theatre and communication studies and received a certificate in musical theatre.

She returned to the east coast to pursue a career in stage and film. Jennie has had a versatile professional stage career thus far, working Off-Broadway and, in the last few years, in Philadelphia where she is a two time Barrymore Award winner. She has been in the original casts of 4 new plays including the Off-Broadway production of "Suburb" and the Barrymore Award-winning "Baby Case" at the Arden Theatre.