Student Peter Parker is labeled a ""bookworm"" by his peers when he declines their offer of a triple-date in favor of viewing a radiology experiment. Bit by a spider, turning into a superhero, his uncle killed by a thief that Peter let go.. fights crime now in Manhattan.

On his first night at work at the Daily Bugle, Peter overhears talk of a fake medicine racket engineered by a rotund mobster called the Kingpin, whose thugs have a laboratory somewhere where they produce cheap, imitation pharmaceuticals, which are then peddled at gunpoint to druggists, who are subsequently frightened into selling the fake medicine and not signing a complaint for the police.

A twisted radiation specialist gains illicit dominion over Manhattan's new and only nuclear power plant and uses a special ray to lift Manhattan into the clouds, and unless he is amply paid, granted amnesty from arrest, and permitted to build his own nuclear reactor, he will deactivate the ray and plunge the island by force of gravity back down to Earth.

Roy Robinson, star football player, campus ladies' man, son of a wealthy chemical industrialist, is envied by Peter, who decides to use his spider-power to play football and outperform Robinson.

Investigating the disappearance of banks (which have been mechanically submerged to deep inside the Earth), Spidey finds a hole that leads to a maze of underground tunnels, through which Spidey undertakes a perilous journey in search of the lost occupants of the submerged banks, and he comes upon a bizarre city populated by group of Molemen led by a maniac intent on conquest of the ""surface people"", some of whom- the occupants of the banks-the Molemen are holding prisoner.

A baseball diamond and the spherical, baseball-sized Optimo Diamond at the Cosmopolitan Museum are connected in this episode as Peter, about to be the star pitcher in a collegiate baseball game, is obliged to don his Spidey-suit once to stop a rampaging ape at the New York City Zoo and again to thwart men in ape disguise at the museum from stealing the Optimo.

Mugs Riley, evidently escaped from prison, has duped the Molemen into following him again, even though they angrily deposed him in ""Menace From the Bottom of the World"", and arranges for Spiderman to be attached to a building so that the web-swinger will be brought underground along with the building, to face the revenge of Mugs Riley.

Giant, mechanical beetles, formed from mushrooms by sonic impulses transmitted from an organ played by the sinister Dr. Manta (a Rocket Robin Hood villain), are used by Manta to capture and enslave the peace-loving inhabitants of an island to mine a valuable ore.

In ancient Egypt, one of the most aggressive of evil magicians, Kotep, the Scarlet Sorcerer, is defeated in battle with an opponent, and his own demons strike him with a cursing ball of fire that puts him in suspended animation, his mummified remains lasting through the passing centuries and becoming an exhibit at a New York university and the object of a professor's obsession.

A giant plant escapes from storage in a house belonging to Prof. Smithers, a missing scientist. Spidey goes through a portal (located in the scientist's home) to prehistoric times to find some defense against the plant and meets Smithers, who tells to him that two radium gems in an idol in a nearby city, if ingested by the plant in New York, will disintegrate it.

Pardo is a sorcerer with the ability to transform himself into a giant cat with hypnotic eyes. He lures top New York City citizens and officials to a theatre with the promise of a spectacular show, then releases his feline alter-ego's power upon the hapless audience, intending to divest them of their wealth and sap the souls out of their bodies.

Peter is an exchange student in South America, flying in a charter airplane with a Latin American professor in the Andes mountains. The airplane encounters a violent storm and crashes in a jungle, and Peter changes to Spiderman to assist the professor and the airplane's two pilots in leaving the jungle and returning to civilization.

At a coffee house, Peter meets Carol, a girl with whom he has much in common; he does not realize to what extent that they are alike until he, as Spiderman, catches Carol in the act of robbing electrical equipment and finds that she has powers identical to his!

Clive, an unbalanced movie producer, is determined to avenge himself upon critics and audiences who spurned his claim that the darkest human emotions could be filmed and physically released from a theatre screen.

A giant Martian warrior, who throws lightning bolts, comes to Earth to rob the planet of its gold. Spiderman's effort to stop the behemoth alien is thwarted by a thieving bomber whom Spidey was about to capture before the Martian appeared on Earth.

Dr. Irving Caldwell has devised a helmet capable of levitating its wearer and is kidnaped by a villainous scientist- the Chinese genius, Dr. Zap, who wants Caldwell's helmet to duplicate for his own evil use.

Sophisticated diamond thief Dr. Cool and his henchman have heisted a fortune in diamonds and are at a deserted-before-dawn ice factory, planning to smuggle the gems through international customs by mixing the ""hot ice"" with large quantities of the ordinary variety.

Two robbers dynamite a bank safe and abscond in their car with millions of stolen dollars. Spidey chases them. The villains throw a ""Vibrator"" device at the web-swinger, and it explodes in Spidey's face! Spidey falls more than 20 building stories to strike a Manhattan street's pavement..