Series Cast 22

1. 

   Josh Keaton

   Peter Parker / Spider Man (voice) (13 Episodes)

2. 

   Joshua LeBar

   Flash Thompson / Car Thief #1 (voice) (1 Episode), Flash Thompson (voice) (11 Episodes)

3. 

   Lacey Chabert

   Gwen Stacy (voice) (12 Episodes)

4. 

   Grey DeLisle

   Betty Brant (voice) (3 Episodes), Sally Avril / Computer Voice (voice) (1 Episode), Sally Avril (voice) (2 Episodes), Sally Avril / Betty Brant (voice) (2 Episodes), Sally Avril / Coffee Clerk (voice) (1 Episode), Sally Avril / Bette Brant (voice) (1 Episode)

5. 

   Deborah Strang

   May Parker (voice) (10 Episodes)

6. 

   Phil LaMarr

   Rand Robertson / Robbie Robertson (voice) (4 Episodes), Rand Robertson / Fancy Dan (voice) (1 Episode), Rand Robertson (voice) (4 Episodes)

7. 

   Ben Diskin

   Eddie Brock (voice) (7 Episodes), Eddie Brock / Venom (voice) (2 Episodes)

8. 

   James Arnold Taylor

   Harry Osborn / Frederick Foswell (voice) (3 Episodes), Harry Osborn (voice) (6 Episodes)

9. 

   Daran Norris

   J. Jonah Jameson / John Jameson (voice) (2 Episodes), J. Jonah Jameson (voice) (6 Episodes)

10. 

    Kevin Michael Richardson

    Big Man / Tombstone / L. Thompson Lincoln (voice) (1 Episode), Big Man / Coach Smith / Thug (voice) (1 Episode), Tombstone / Principal Davis (voice) (1 Episode), Tombstone (voice) (3 Episodes), Coach Smith (voice) (1 Episode)

11. 

    John DiMaggio

    Flint Marko / Hammerhead (voice) (2 Episodes), Sandman / Hammerhead (voice) (1 Episode), Flint Marko / Sandman / Hammerhead (voice) (1 Episode), Hammerhead / Car Thief #2 (voice) (1 Episode), Hammerhead (voice) (2 Episodes)

12. 

    Clancy Brown

    Alex O'Hirn / Rhino / George Stacy (voice) (1 Episode), Alex O'Hirn / Ox (voice) (1 Episode), George Stacy (voice) (2 Episodes), Rhino / George Stacy (voice) (1 Episode), Alex O'Hirn (voice) (2 Episodes)

13. 

    Vanessa Marshall

    Mary Jane Watson (voice) (7 Episodes)

14. 

    Alan Rachins

    Norman Osborn (voice) (7 Episodes)

15. 

    Peter MacNicol

    Otto Octavius / Doctor Octopus (voice) (1 Episode), Otto Octavius (voice) (4 Episodes), Doctor Octopus (voice) (1 Episode)

16. 

    Alanna Ubach

    Liz Allan (voice) (6 Episodes)

17. 

    Dee Bradley Baker

    Curt Connors / Lizard (voice) (1 Episode), Curt Connors (voice) (3 Episodes)

18. 

    Crispin Freeman

    Max Dillon / Electro (voice) (1 Episode), Electro (voice) (1 Episode), Thug #1 (voice) (1 Episode)

19. 

    Cree Summer

    Glory Grant (voice) (2 Episodes)

20. 

    Robert Englund

    Adrian Toomes / Vulture (voice) (1 Episode), Vulture (voice) (1 Episode)

21. 

    Ed Asner

    Ben Parker (voice) (1 Episode)

22. 

    Tricia Helfer

    Black Cat (voice) (1 Episode)

Series Crew 13