Series Cast 24

1. 

   Ben Diskin

   Flash Thompson (25 Episodes), Police Officer #2/Hoodlum #1/Police Officer #4 (voice) (1 Episode), Osborn Recruiter #1 (voice) (1 Episode), Flash Thompson (voice) (1 Episode), Student #2 (voice) (1 Episode)

2. 

   Melanie Minichino

   Anya Corazon (voice) (25 Episodes), Chaperone (voice) (1 Episode), Helicopter Pilot (voice) (1 Episode), Student #1 (voice) (1 Episode), Female Clerk (voice) (1 Episode)

3. 

   Fred Tatasciore

   Trucker/Mr. Slott (voice) (1 Episode), Police Officer #1 (voice) (1 Episode), Bystander (voice) (1 Episode), Thug #1 / Father (voice) (1 Episode), Max Modell (voice) (25 Episodes)

4. 

   Nancy Linari

   Woman Bystander (voice) (1 Episode), Bomb Voice (voice) (1 Episode), Landlady (voice) (1 Episode), Aunt May (voice) (25 Episodes)

5. 

   Max Mittelman

   Police Officer #1/Osborn Technician #2/Police Officer #2 (voice) (1 Episode), Male Clerk/Guy in Line (voice) (1 Episode), Thug #2 / Little Kid (voice) (1 Episode), Harry Osborn (voice) (25 Episodes)

6. 

   Nadji Jeter

   Miles Morales (voice) (25 Episodes), Security Robot (voice) (2 Episodes)

7. 

   John DiMaggio

   Security Guard/Customer (voice) (1 Episode), Policeman (voice) (1 Episode), Raymond Warren (voice) (25 Episodes)

8. 

   Josh Keaton

   Prison Guard (voice) (1 Episode), Norman Osborn (voice) (25 Episodes), Hologram (voice) (1 Episode)

9. 

   Laura Bailey

   Gwen Stacy (voice) (25 Episodes), Osborn Recruiter #2 (voice) (1 Episode)

10. 

    Alastair Duncan

    Adrian Toomes (voice) (25 Episodes), Thug #3 (voice) (1 Episode)

11. 

    Robbie Daymond

    Peter Parker / Spider-Man (voice) (25 Episodes)

12. 

    Patton Oswalt

    Uncle Ben (voice) (25 Episodes)

13. 

    Scott Menville

    Doc Ock / Otto Octavius (voice) (25 Episodes)

14. 

    Jim Cummings

    Hammerhead (voice) (25 Episodes)

15. 

    Jason Spisak

    Alistair Smythe (voice) (3 Episodes), Audience Member (voice) (1 Episode), Alistair Smythe / Scorpion (voice) (1 Episode)

16. 

    Matthew Mercer

    Aleksei Sytsevich/Comic Book Guy (voice) (1 Episode), Aleksei Sytsevich / Rhino / Reporter (voice) (1 Episode)

17. 

    Sofia Carson

    Sand Girl (voice) (1 Episode), Keemia (voice) (1 Episode)

18. 

    Cameron Boyce

    Herman Schultz / Shocker (voice) (1 Episode)

19. 

    Richard Doyle

    Police Officer #3 (voice) (1 Episode)

20. 

    Zeno Robinson

    Randy Robertson (voice) (1 Episode)

21. 

    Daniel Hagen

    Cop (voice) (1 Episode)

22. 

    Yuri Lowenthal

    Clayton Cole / Clash (voice) (1 Episode)

23. 

    Grey DeLisle

    Black Cat/Nurse/Mom (voice) (1 Episode)

24. 

    Travis Willingham

    Sandman / Flint Marko (voice) (1 Episode)

Series Crew 16