Elenco Principal da Temporada 8

1. 

   Christopher Daniel Barnes

   Peter Parker / Spider-Man (voice)

2. 

   Sara Ballantine

   Mary Jane Watson (voice)

3. 

   Jennifer Hale

   Felicia Hardy (voice)

4. 

   Linda Gary

   Aunt May Parker (voice)

5. 

   Gary Imhoff

   Harry Osborn (voice)

6. 

   Ed Asner

   J. Jonah Jameson (voice)

7. 

   Rodney Saulsberry

   Joe "Robbie" Robertson (voice)

8. 

   Roscoe Lee Browne

   Kingpin (voice)

Convidados Especiais 12

1. 

   Joseph Campanella

   Dr. Curt Connors (voice)

2. John Beard

   Newscaster (voice)

3. 

   Hank Azaria

   Eddie Brock / Venom (voice)

4. 

   Ed Gilbert

   Dormammu (voice)

5. 

   Scott Cleverdon

   Cletus Kasady / Carnage (voice)

6. 

   James Avery

   Jim Rhodes / War Machine (voice)

7. 

   Barbara Goodson

   Dr. Ashley Kafka (voice)

8. 

   Dawnn Lewis

   Detective Terri Lee (voice)

9. Liz Georges

   Debra Whitman (voice)

10. 

    Robert Hays

    Tony Stark / Iron Man (voice)

11. 

    Tony Jay

    Baron Mordo (voice)

12. 

    Joan Lee

    Madame Web (voice)

Equipa Técnica 4